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dark field microscope

What is CytoViva® Enhanced dark field microscope Optics?

What is CytoViva® Enhanced dark field microscope Optics?

CytoViva’s enhanced darkfield microscope optics improve signal-to-noise up to ten times (10x) over standard darkfield optics1. This enables nanomaterials as small as 10nm-20nm to be imaged right from your laboratory benchtop2.

CytoViva’s patented (US patents No. 7,542,203, 7,564,623) enhanced darkfield illumination system, which replaces the standard microscope condenser, works by coupling the source illumination directly to the condenser optics. In this optical path, collimating lenses and mirrors align and fix the geometry of the light to match the geometry of the condenser annulus. This creates a very narrow, oblique angle of source illumination that can be precisely focused into the sample but bypasses the objective. The result is very intense scatter from nanoscale samples against a very dark background. Source illumination compatible with this system can be halogen, xenon or even laser, depending on the application.

Enhanced Darkfield Illumination Optics

CytoViva’s enhanced darkfield optics enable scientists to optically observe a wide range of nanoscale materials quickly and easily in solution, live cells, tissue and materials based matrices. In addition, non-fluorescent live cells and pathogens can be easily observed at a level of detail not possible with traditional optical imaging techniques such as phase contrast or differential interference contrast.

Finally, when combined with CytoViva’s Hyperspectral Imaging capability this high signal-to-noise microscopy method enables researchers to spectrally characterize and map nanoscale samples in a wide range of environments.

To see just how easy CytoViva is to use, simply watch this brief video overview of the installation and alignment process.
Please email sales@CytoViva.com to request your private web demonstration.

dark field microscope

What Different dark field from conventional microscopy?

What Different dark field from conventional microscopy?

In conventional bright field illumination, your specimen is lit from a central light source (you can read more about bright field microscopy in this Bitesize Bio article). This results in a large contrast image. However, in dark field microscope this light source is blocked by a condenser or a ‘stop’ below the stage. This condenser or stop scatters the light allowing only oblique rays to reflect and refract off your specimen which in turn creates a bright image on a dark background.

dark field microscope

What is Conclusion about dark field microscope?

A dark field microscope can offer brilliant, light images against a dark background of otherwise difficult to view specimens.

Most standard microscopes come with dark field capabilities or accessories to enable this illumination technique.

There are many practical applications of dark field, especially in the field of marine biology, in viewing the many specimens you cannot see using alternative techniques.

However, a researcher must keep in mind the potential issues and limitations that may arise from dark field illumination.

For further information, check out the many microscopy imaging techniques available.

dark field microscope

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