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dark field microscopy uses

What Advantages and Disadvantages about dark field microscopy uses?

No one system is perfect, and dark field microscopy may or may not appeal to you depending on your needs.

Some advantages of using a dark field microscope are:

Extremely simple to use
Inexpensive to set up (instructions on how to make your own dark field microscope are below)
Very effective in showing the details of live and unstained samples
Some of the disadvantages are:

Limited colors (certain colors will appear, but they’re less accurate and most images will be just black and white)
Images can be difficult to interpret to those unfamiliar with dark field microscopy
Although surface details can be very apparent, the internal details of a specimen often don’t stand out as much with a dark field setup.

Below are contrasting examples of dark field (left) versus bright field (right) illumination of lens tissue paper. Note how they both create a different style of image.

Dark field illumination Bright field illumination

Admit it, by now you’re curious to check out your own dark field! You can create one with minimal time and effort. Just read on…

dark field microscopy uses

dark field microscopy uses TECHNOLOGY:

dark field microscopy uses TECHNOLOGY:

Darkfield microscopy creates contrast in transparent unstained specimens such as living cells. It depends on controlling specimen illumination so that central light which normally passes through and around the specimen is blocked. Rather than light illuminating the sample with a full cone of light (as in brightfield microscopy) the condenser forms a hollow cone with light travelling around the cone rather than through it.

This form of illumination allows only oblique rays of light to strike the specimen on the microscope stage and the image is formed by rays of light scattered by the sample and captured in the objective lens. When there is no sample on the microscope stage the view is completely dark.

Care should be taken in preparing specimens as features above and below the plane of focus can also scatter light and compromise image quality (for example, dust, fingerprints). In general, thin specimens are better because the possibility of diffraction artifacts is reduced.

dark field microscopy uses

What PARTS of dark field microscopy uses?

What PARTS of dark field microscopy uses?

These are three requisites for adopting an ordinary microscope for Dark ground illumination:
1 Dark ground condenser
2 Suitable light intensity lamp
3 Funnel stop (to reduce numerical aperture of objective)
USES:
1. To visualize Trepenoma pallidum in exudates.
2. To visualize Leptospira species in blood & urine.
3. To visualize Spirillum minus in blood.
4. To detect motility of other bacteria.

5. To identify sheathed microfilaria & other parasites

dark field microscopy uses

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