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The Truth About dark field microscopy spirochetes

dark field microscopy spirochetes

What is CytoViva® Enhanced dark field microscopy spirochetes Optics?

What is CytoViva® Enhanced dark field microscopy spirochetes Optics?

CytoViva’s enhanced darkfield microscope optics improve signal-to-noise up to ten times (10x) over standard darkfield optics1. This enables nanomaterials as small as 10nm-20nm to be imaged right from your laboratory benchtop2.

CytoViva’s patented (US patents No. 7,542,203, 7,564,623) enhanced darkfield illumination system, which replaces the standard microscope condenser, works by coupling the source illumination directly to the condenser optics. In this optical path, collimating lenses and mirrors align and fix the geometry of the light to match the geometry of the condenser annulus. This creates a very narrow, oblique angle of source illumination that can be precisely focused into the sample but bypasses the objective. The result is very intense scatter from nanoscale samples against a very dark background. Source illumination compatible with this system can be halogen, xenon or even laser, depending on the application.

Enhanced Darkfield Illumination Optics

CytoViva’s enhanced darkfield optics enable scientists to optically observe a wide range of nanoscale materials quickly and easily in solution, live cells, tissue and materials based matrices. In addition, non-fluorescent live cells and pathogens can be easily observed at a level of detail not possible with traditional optical imaging techniques such as phase contrast or differential interference contrast.

Finally, when combined with CytoViva’s Hyperspectral Imaging capability this high signal-to-noise microscopy method enables researchers to spectrally characterize and map nanoscale samples in a wide range of environments.

To see just how easy CytoViva is to use, simply watch this brief video overview of the installation and alignment process.
Please email sales@CytoViva.com to request your private web demonstration.

dark field microscopy spirochetes

When to Use a dark field microscopy spirochetese

When to Use a dark field microscopy spirochetese

Dark field microscopes are used in a number of different ways to view a variety of specimens that are hard to see in a light field unit. Live bacteria, for example, are best viewed with this type of microscope, as these organisms are very transparent when unstained.

There are multitudes of other ways to use dark field illumination, often when the specimen is clear or translucent. Some examples:

Dark field illumination of caffeine crystalsLiving or lightly stained transparent specimens

Single-celled organisms

Live blood samples

Aquatic environment samples (from seawater to pond water)

Living bacteria

Hay or soil samples

Pollen samples

Certain molecules such as caffeine crystals (right)

Dark field microscopy makes many invisible specimens appear visible. Most of the time the specimens invisible to bright field illumination are living, so you can see how important it is to bring them into view!

dark field microscopy spirochetes

What is dark field microscopy spirochetes?

What is dark field microscopy spirochetes?

Dark Field microscopy is a microscope illumination technique used to observe unstained samples causing them to appear brightly lit against a dark, almost purely black, background.

When light hits an object, rays are scattered in all directions. The design of the dark field microscope is such that it removes the dispersed light so that only the scattered beams hit the sample.

The introduction of a condenser and/or stop below the stage ensures that these light rays will hit the specimen at different angles, rather than as a direct light source above/below the object.

The result is a “cone of light” where rays are diffracted, reflected and/or refracted off the object, ultimately, allowing you to view a specimen in dark field.

A dark field microscope is ideal for viewing objects that are unstained, transparent and absorb little or no light.

These specimens often have similar refractive indices as their surroundings, making them hard to distinguish with other illumination techniques.

Dark field can be used to study marine organisms such as algae and plankton, diatoms, insects, fibres, hairs, yeast, live bacterium, protozoa as well as cells and tissues and is ideal for live blood analysis enabling the practitioner to see much more than is possible with other lighting methods.

dark field microscopy spirochetes

What Different dark field from conventional microscopy?

What Different dark field from conventional microscopy?

In conventional bright field illumination, your specimen is lit from a central light source (you can read more about bright field microscopy in this Bitesize Bio article). This results in a large contrast image. However, in dark field microscopy spirochetes this light source is blocked by a condenser or a ‘stop’ below the stage. This condenser or stop scatters the light allowing only oblique rays to reflect and refract off your specimen which in turn creates a bright image on a dark background.

dark field microscopy spirochetes

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