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microscope dark field

What Advantages and Disadvantages about microscope dark field?

What Advantages and Disadvantages about microscope dark field?

No one system is perfect, and dark field microscopy may or may not appeal to you depending on your needs.

Some advantages of using a dark field microscope are:

Extremely simple to use
Inexpensive to set up (instructions on how to make your own dark field microscope are below)
Very effective in showing the details of live and unstained samples
Some of the disadvantages are:

Limited colors (certain colors will appear, but they’re less accurate and most images will be just black and white)
Images can be difficult to interpret to those unfamiliar with dark field microscopy
Although surface details can be very apparent, the internal details of a specimen often don’t stand out as much with a dark field setup.

Below are contrasting examples of dark field (left) versus bright field (right) illumination of lens tissue paper. Note how they both create a different style of image.

Dark field illumination Bright field illumination

Admit it, by now you’re curious to check out your own dark field! You can create one with minimal time and effort. Just read on…

microscope dark field

What PARTS of microscope dark field?

These are three requisites for adopting an ordinary microscope for microscope dark field:
1 Dark ground condenser
2 Suitable light intensity lamp
3 Funnel stop (to reduce numerical aperture of objective)
1. To visualize Trepenoma pallidum in exudates.
2. To visualize Leptospira species in blood & urine.
3. To visualize Spirillum minus in blood.
4. To detect motility of other bacteria.

5. To identify sheathed microfilaria & other parasites

microscope dark field

What is microscope dark field?

dark field microscopy of sugar crystals
Dark Field illumination is a technique used to observe unstained samples causing them to appear brightly lit against a dark, almost purely black, background.

Pictured right: Highly magnified image of sugar crystals using darkfield microscopy technique

When light hits an object, rays are scattered in all azimuths or directions. The design of the dark field microscope is such that it removes the dispersed light, or zeroth order, so that only the scattered beams hit the sample.

The introduction of a condenser and/or stop below the stage ensures that these light rays will hit the specimen at different angles, rather than as a direct light source above/below the object.

The result is a “cone of light” where rays are diffracted, reflected and/or refracted off the object, ultimately, allowing you to view a specimen in dark field.

microscope dark field

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