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Why I’m no longer satisfied with dark field microscopy ppt — and what I’m doing about it.

dark field microscopy ppt

dark field microscopy ppt lyme disease

Although this video clearly shows a spirochaetal shaped bacteria, it cannot identify what spirochaete it may be. To show that those bacteria are in fact Borrelia (the spirochaetal bacteria shown to cause Lyme borreliosis), proper DNA sequencing would have to occur. From our perspective, if the person is sick and these are showing up in their blood, then treat the patient for a spirochaetosis while waiting for confirmation. Treatment is chosen within the patient/doctor discussion as to type of antibiotic and duration. Infectious Disease Society of America (IDSA) guidelines are not recommended. Current Canadian medical policy is to simply defer to the IDSA guidelines on matters of testing and treatment therefore we cannot recommend following Canadian medical policy in any province until patient expert’s opinion is given full and equal voice in the writing of Canadian medical policy relative to Lyme borreliosis.]

Slide 5

These video clips are from an experiment in which 11 friends provided a tiny amount of fingertip blood on a microscope slide.

All donors were met through patient support groups and have chronic illness.

8 of 11 have been ill for 20 years or longer.

9 have been diagnosed with M.E. or Chronic Fatigue Syndrome

10 of 11 had negative NHS tests for Lyme borreliosis – one was not tested.

9 had private tests that were positive.

These eleven donors represent a total of 235 years of illness and 170
years of lost productivity.

dark field microscopy ppt

What is CytoViva® Enhanced dark field microscopy ppt Optics?

What is CytoViva® Enhanced dark field microscopy ppt Optics?

CytoViva’s enhanced darkfield microscope optics improve signal-to-noise up to ten times (10x) over standard darkfield optics1. This enables nanomaterials as small as 10nm-20nm to be imaged right from your laboratory benchtop2.

CytoViva’s patented (US patents No. 7,542,203, 7,564,623) enhanced darkfield illumination system, which replaces the standard microscope condenser, works by coupling the source illumination directly to the condenser optics. In this optical path, collimating lenses and mirrors align and fix the geometry of the light to match the geometry of the condenser annulus. This creates a very narrow, oblique angle of source illumination that can be precisely focused into the sample but bypasses the objective. The result is very intense scatter from nanoscale samples against a very dark background. Source illumination compatible with this system can be halogen, xenon or even laser, depending on the application.

Enhanced Darkfield Illumination Optics

CytoViva’s enhanced darkfield optics enable scientists to optically observe a wide range of nanoscale materials quickly and easily in solution, live cells, tissue and materials based matrices. In addition, non-fluorescent live cells and pathogens can be easily observed at a level of detail not possible with traditional optical imaging techniques such as phase contrast or differential interference contrast.

Finally, when combined with CytoViva’s Hyperspectral Imaging capability this high signal-to-noise microscopy method enables researchers to spectrally characterize and map nanoscale samples in a wide range of environments.

To see just how easy CytoViva is to use, simply watch this brief video overview of the installation and alignment process.
Please email sales@CytoViva.com to request your private web demonstration.

dark field microscopy ppt

What is dark field microscopy ppt?

What is dark field microscopy ppt?

Bright-field microscopy is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample. Bright-field microscopy is the simplest of a range of techniques used for illumination of samples in light microscopes, and its simplicity makes it a popular technique. The typical appearance of a bright-field microscopy image is a dark sample on a bright background, hence the name.

dark field microscopy ppt Light path

The light path of a bright-field microscope is extremely simple, no additional components are required beyond the normal light-microscope setup. The light path therefore consists of:

a transillumination light source, commonly a halogen lamp in the microscope stand;
a condenser lens, which focuses light from the light source onto the sample;
an objective lens, which collects light from the sample and magnifies the image;
oculars and/or a camera to view the sample image.

Bright-field microscopy may use critical or Köhler illumination to illuminate the sample.

dark field microscopy ppt Performance

Bright-field microscopy typically has low contrast with most biological samples, as few absorb light to a great extent. Staining is often required to increase contrast, which prevents use on live cells in many situations. Bright-field illumination is useful for samples that have an intrinsic color, for example chloroplasts in plant cells.Bright-field microscopy is a standard light-microscopy technique, and therefore magnification is limited by the resolving power possible with the wavelength of visible light.

dark field microscopy ppt Advantages

Simplicity of setup with only basic equipment required.
Living cells can be seen with bright-field microscopes

dark field microscopy ppt Limitations

Very low contrast of most biological samples.
The practical limit to magnification with a light microscope is around 1300X. Although higher magnifications are possible, it becomes increasingly difficult to maintain image clarity as the magnification increases.
Low apparent optical resolution due to the blur of out-of-focus material.
Samples that are naturally colorless and transparent cannot be seen well, e.g. many types of mammalian cells. These samples often have to be stained before viewing. Samples that do have their own color can be seen without preparation, e.g. the observation of cytoplasmic streaming in Chara cells.

dark field microscopy ppt Enhancements

Reducing or increasing the amount of the light source by the iris diaphragm.
Use of an oil-immersion objective lens and a special immersion oil placed on a glass cover over the specimen. Immersion oil has the same refraction as glass and improves the resolution of the observed specimen.
Use of sample-staining methods for use in microbiology, such as simple stains (methylene blue, safranin, crystal violet) and differential stains (negative stains, flagellar stains, endospore stains).
Use of a colored (usually blue) or polarizing filter on the light source to highlight features not visible under white light. The use of filters is especially useful with mineral samples.

dark field microscopy ppt

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