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bright field and dark field imaging

How to change Dark Field Transformation?

How to change Dark Field Transformation?

Most stereo and standard compound microscopes have the potential for dark field microscopy.

If a microscope has built-in elements to easily modify for dark field illumination, the manufacturer usually lists this amongst the observation specifications.

You can achieve dark field by using condensers, mirrors and/or a “stop.” Some microscopes come with these accessories or researchers can purchase dark field kits, or even use some common items to adapt a microscope for dark field illumination.

In bright field illumination, the object is lit from below the stage, resulting in a larger, contrasted image that can be studied.

A dark field microscope blocks this central light with a condenser so that only oblique rays hit the object.

An Abbe condenser, for example, contains a concave orb that collects light rays in all azimuths that bounce off a sample to form a cone of illumination.

If there is nothing on the stage, the aperture of the condenser is greater than the objective and the view will be completely black.

A stop is an opaque object that blocks the central light when placed underneath the stage condenser.

This also causes light to scatter in all azimuths, resulting in a cone of light that allows for dark field observation.

Too expensive? What you can do…

If you do not have access to these accessories and cannot afford a dark field kit, there are alternative ways to adapt your microscope for dark field illumination.

The expensive stops are all made of opaque material.

Any possible substitutions cannot have any transparent properties.

One option is to use a circular object, such as a coin; adhere the coin to a larger disk and place below the stage.

You can also cut out a round piece of thick paper, such as construction paper, cardboard or poster-board, and attach to the condenser.

Whatever you use, the trick is to find the right diameter so that the makeshift stop will block the light and only allow the oblique rays to illuminate the specimen.

bright field and dark field imaging

bright field and dark field imaging advantages

bright field and dark field imaging advantages

No one system is perfect, and bright field and dark field imaging may or may not appeal to you depending on your needs.

Some advantages of using a dark field microscope are:

Extremely simple to use

Inexpensive to set up (instructions on how to make your own dark field microscope are below)

Very effective in showing the details of live and unstained samples

bright field and dark field imaging

What is CytoViva® Enhanced bright field and dark field imaging Optics?

What is CytoViva® Enhanced bright field and dark field imaging Optics?

CytoViva’s enhanced darkfield microscope optics improve signal-to-noise up to ten times (10x) over standard darkfield optics1. This enables nanomaterials as small as 10nm-20nm to be imaged right from your laboratory benchtop2.

CytoViva’s patented (US patents No. 7,542,203, 7,564,623) enhanced darkfield illumination system, which replaces the standard microscope condenser, works by coupling the source illumination directly to the condenser optics. In this optical path, collimating lenses and mirrors align and fix the geometry of the light to match the geometry of the condenser annulus. This creates a very narrow, oblique angle of source illumination that can be precisely focused into the sample but bypasses the objective. The result is very intense scatter from nanoscale samples against a very dark background. Source illumination compatible with this system can be halogen, xenon or even laser, depending on the application.

Enhanced Darkfield Illumination Optics

CytoViva’s enhanced darkfield optics enable scientists to optically observe a wide range of nanoscale materials quickly and easily in solution, live cells, tissue and materials based matrices. In addition, non-fluorescent live cells and pathogens can be easily observed at a level of detail not possible with traditional optical imaging techniques such as phase contrast or differential interference contrast.

Finally, when combined with CytoViva’s Hyperspectral Imaging capability this high signal-to-noise microscopy method enables researchers to spectrally characterize and map nanoscale samples in a wide range of environments.

To see just how easy CytoViva is to use, simply watch this brief video overview of the installation and alignment process.
Please email sales@CytoViva.com to request your private web demonstration.

bright field and dark field imaging

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