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The dark field objective guide to dark field objective -ing.

dark field objective

What Makes Up Healthy Blood?

What one sees in the mobile situation are the usual red blood cells, white blood cells, plasma—and what is floating in the plasma. Microbial activity, undigested food, fungi, and crystals are all apparent as is the capacity of the red blood cells to circulate and the white blood cells to devour morbid matter.

(Live Blood Examination in the Darkfield according to Prof. Dr. G. Enderlein)
dark field objective or Live Blood Analysis is a way of studying live whole blood cells under a specially adapted microscope that projects the dynamic image onto a video screen. This allows you to view your inner terrain. Digestive, eliminative and immune functions can be assessed as well as the presence of bacteria and other micro-organisms.

The darkfield microscopic examination of the freshly taken live blood is one of the most important examinations of the holistic medicine applied at the Centre. It enables us to view the inner terrain (milieu) and to examine the functions of the red blood cells. It also shows the evolutionary stages of the smallest proteins (endobionts) which are found in every human body. We are also able to see any developed structures such as bacteria, virus and fungus. The darkfield examination shows the state of the blood cells, endobionts and the plasma in a functional and structural way, making bacterial processes and fungal pre-stages in the blood clearly visible.

The darkfield examination is most suitable for the evaluation of chronic diseases; for children who are prone to infections; for recurrent bacterial problems; for candida and other fungal problems and also to answer questions concerning chronic problems of toxicity (e.g. amalgam disturbances).

dark field objective

What is Conclusion about dark field objective

A dark field objective can offer brilliant, light images against a dark background of otherwise difficult to view specimens.

Most standard microscopes come with dark field capabilities or accessories to enable this illumination technique.

There are many practical applications of dark field, especially in the field of marine biology, in viewing the many specimens you cannot see using alternative techniques.

However, a researcher must keep in mind the potential issues and limitations that may arise from dark field illumination.

For further information, check out the many microscopy imaging techniques available.

dark field objective

What Disadvantages of dark field objective

What Disadvantages of dark field objective

A dark field microscope can result in beautiful and amazing images; this technique also comes with a number of disadvantages.

First, dark field images are prone to degradation, distortion and inaccuracies.
A specimen that is not thin enough or its density differs across the slide, may appear to have artifacts throughout the image.
The preparation and quality of the slides can grossly affect the contrast and accuracy of a dark field image.
You need to take special care that the slide, stage, nose and light source are free from small particles such as dust, as these will appear as part of the image.
Similarly, if you need to use oil or water on the condenser and/or slide, it is almost impossible to avoid all air bubbles.
These liquid bubbles will cause images degradation, flare and distortion and even decrease the contrast and details of the specimen.
Dark field needs an intense amount of light to work. This, coupled with the fact that it relies exclusively on scattered light rays, can cause glare and distortion.
It is not a reliable tool to obtain accurate measurements of specimens.
Finally, numerous problems can arise when adapting and using a dark field microscope. The amount and intensity of light, the position, size and placement of the condenser and stop need to be correct to avoid any aberrations.
Dark field has many applications and is a wonderful observation tool, especially when used in conjunction with other techniques.

However, when employing this technique as part of a research study, you need to take into consideration the limitations and knowledge of possible unwanted artifacts.

dark field objective

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