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bright field and dark field microscopy pdf

What Makes Up Healthy Blood?

What one sees in the mobile situation are the usual red blood cells, white blood cells, plasma—and what is floating in the plasma. Microbial activity, undigested food, fungi, and crystals are all apparent as is the capacity of the red blood cells to circulate and the white blood cells to devour morbid matter.

(Live Blood Examination in the Darkfield according to Prof. Dr. G. Enderlein)
bright field and dark field microscopy pdf or Live Blood Analysis is a way of studying live whole blood cells under a specially adapted microscope that projects the dynamic image onto a video screen. This allows you to view your inner terrain. Digestive, eliminative and immune functions can be assessed as well as the presence of bacteria and other micro-organisms.

The darkfield microscopic examination of the freshly taken live blood is one of the most important examinations of the holistic medicine applied at the Centre. It enables us to view the inner terrain (milieu) and to examine the functions of the red blood cells. It also shows the evolutionary stages of the smallest proteins (endobionts) which are found in every human body. We are also able to see any developed structures such as bacteria, virus and fungus. The darkfield examination shows the state of the blood cells, endobionts and the plasma in a functional and structural way, making bacterial processes and fungal pre-stages in the blood clearly visible.

The darkfield examination is most suitable for the evaluation of chronic diseases; for children who are prone to infections; for recurrent bacterial problems; for candida and other fungal problems and also to answer questions concerning chronic problems of toxicity (e.g. amalgam disturbances).

bright field and dark field microscopy pdf

What Principles of bright field and dark field microscopy pdf?

To view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scattered by objects on the slide can reach the eye. Instead of coming up through the specimen, the light is reflected by particles on the slide. Everything is visible regardless of color, usually bright white against a dark background.

Pigmented objects are often seen in “false colors,” that is, the reflected light is of a color different than the color of the object. Better resolution can be obtained using dark as opposed to bright field viewing.

Sophisticated equipment is not necessary to get a dark field effect, but you do need a higher intensity light, since you are seeing only reflected light. At low magnification (up to 100x) any decent optical instrument can be set up so that light is reflected toward the viewer rather than passing through the object directly toward the viewer.

bright field and dark field microscopy pdf

What is CytoViva® Enhanced bright field and dark field microscopy pdf Optics

 

CytoViva’s enhanced darkfield microscope optics improve signal-to-noise up to ten times (10x) over standard darkfield optics1.  This enables nanomaterials as small as 10nm-20nm to be imaged right from your laboratory benchtop2.

CytoViva’s patented (US patents No. 7,542,203, 7,564,623) enhanced darkfield illumination system, which replaces the standard microscope condenser, works by coupling the source illumination directly to the condenser optics. In this optical path, collimating lenses and mirrors align and fix the geometry of the light to match the geometry of the condenser annulus. This creates a very narrow, oblique angle of source illumination that can be precisely focused into the sample but bypasses the objective. The result is very intense  scatter from nanoscale samples against a very dark background.  Source illumination compatible with this system can be halogen, xenon or even laser, depending on the application.

Enhanced Darkfield Illumination Optics

CytoViva’s enhanced darkfield optics enable scientists to optically observe a wide range of nanoscale materials quickly and easily in solution, live cells, tissue and materials based matrices. In addition, non-fluorescent live cells and pathogens can be easily observed at a level of detail not possible with traditional optical imaging techniques such as phase contrast or differential interference contrast.

Finally, when combined with CytoViva’s Hyperspectral Imaging capability this high signal-to-noise microscopy method enables researchers to spectrally characterize and map nanoscale samples in a wide range of environments.

To see just how easy CytoViva is to use, simply watch this brief video overview of the installation and alignment process.
Please email sales@CytoViva.com to request your private web demonstration.

bright field and dark field microscopy pdf

What PARTS of bright field and dark field microscopy pdf?

These are three requisites for adopting an ordinary microscope for bright field and dark field microscopy pdf:
1 Dark ground condenser
2 Suitable light intensity lamp
3 Funnel stop (to reduce numerical aperture of objective)
USES:
1. To visualize Trepenoma pallidum in exudates.
2. To visualize Leptospira species in blood & urine.
3. To visualize Spirillum minus in blood.
4. To detect motility of other bacteria.

5. To identify sheathed microfilaria & other parasites

bright field and dark field microscopy pdf

bright field and dark field microscopy pdf APPLICATIONS

 

• Viewing blood cells (biological dark field microscope, combined with phase contrast)
• Viewing bacteria (biological dark field microscope, often combined with phase contrast)
• Viewing different types of algae (biological dark field microscope)
• Viewing hairline metal fractures (metallurgical dark field microscope)
• Viewing diamonds and other precious stones (gemological microscope or stereo dark field microscope)
• Viewing shrimp or other invertebrates (stereo dark field microscope)

bright field and dark field microscopy pdf

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