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Why being dark field blood test is a surprisingly good idea

What is dark field blood test?

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Thus, the dark field blood analysis provides information about the function and structure of blood cells and plasma endobionts, as well as developing bacterial and fungal precursors. The dark field examination also indicates changes within the cell through hormonal and mineral deficiencies. It is particularly beneficial for the evaluation of patients with chronic diseases, and children with susceptibility to infection, or recurrent bacterial problems, such as Candida or other fungal diseases. Dark field blood analysis is crucial in answering questions related to any chronic, or toxic problems.

Dark field microscopy is also an important tool in biological therapies. It can be used to test the effect of certain medications, by adding the medication to a blood sample and analyzing the reaction that is produced. This investigation is extremely motivating for the patient, by allowing him to directly experience the diagnosis.

The effects of dark field microscopy can not be replaced by any other blood test, especially not by normal laboratory microscopic blood tests, sent in fixed samples, as the blood changes its function due to environmental changes, so the blood must be tested while it is still fresh. It is also important to evaluate the degeneration tendency of blood samples, especially when considering the behavior of tumors.

The presence of bacteria precursors, which are not disease-inducing, but increase the risk of future disease development, can also be found in the dark field examination. Therefore, this dark field examination of the blood is a valuable and necessary preventative measure.

What Makes Up Healthy Blood?

What one sees in the mobile situation are the usual red blood cells, white blood cells, plasma—and what is floating in the plasma. Microbial activity, undigested food, fungi, and crystals are all apparent as is the capacity of the red blood cells to circulate and the white blood cells to devour morbid matter.

(Live Blood Examination in the Darkfield according to Prof. Dr. G. Enderlein)
Darkfield Microscopy or Live Blood Analysis is a way of studying live whole blood cells under a specially adapted microscope that projects the dynamic image onto a video screen. This allows you to view your inner terrain. Digestive, eliminative and immune functions can be assessed as well as the presence of bacteria and other micro-organisms.

The darkfield microscopic examination of the freshly taken live blood is one of the most important examinations of the holistic medicine applied at the Centre. It enables us to view the inner terrain (milieu) and to examine the functions of the red blood cells. It also shows the evolutionary stages of the smallest proteins (endobionts) which are found in every human body. We are also able to see any developed structures such as bacteria, virus and fungus. The darkfield examination shows the state of the blood cells, endobionts and the plasma in a functional and structural way, making bacterial processes and fungal pre-stages in the blood clearly visible.

The darkfield examination is most suitable for the evaluation of chronic diseases; for children who are prone to infections; for recurrent bacterial problems; for candida and other fungal problems and also to answer questions concerning chronic problems of toxicity (e.g. amalgam disturbances).

What dark field microscopy ?

Dark-field microscopy (dark-ground microscopy) describes microscopy methods, in both light and electron microscopy, which exclude the unscattered beam from the image. As a result, the field around the specimen (i.e., where there is no specimen to scatter the beam) is generally dark.

Light microscopy applications

In optical microscopy, dark-field describes an illumination technique used to enhance the contrast in unstained samples. It works by illuminating the sample with light that will not be collected by the objective lens and thus will not form part of the image. This produces the classic appearance of a dark, almost black, background with bright objects on it.

The light’s path

The steps are illustrated in the figure where an inverted microscope is used.
Diagram illustrating the light path through a dark-field microscope

Light enters the microscope for illumination of the sample.
A specially sized disc, the patch stop (see figure), blocks some light from the light source, leaving an outer ring of illumination. A wide phase annulus can also be reasonably substituted at low magnification.
The condenser lens focuses the light towards the sample.
The light enters the sample. Most is directly transmitted, while some is scattered from the sample.
The scattered light enters the objective lens, while the directly transmitted light simply misses the lens and is not collected due to a direct-illumination block (see figure).
Only the scattered light goes on to produce the image, while the directly transmitted light is omitted.

Advantages and disadvantages

Dark-field microscopy is a very simple yet effective technique and well suited for uses involving live and unstained biological samples, such as a smear from a tissue culture or individual, water-borne, single-celled organisms. Considering the simplicity of the setup, the quality of images obtained from this technique is impressive.

The main limitation of dark-field microscopy is the low light levels seen in the final image. This means that the sample must be very strongly illuminated, which can cause damage to the sample. Dark-field microscopy techniques are almost entirely free of artifacts, due to the nature of the process. However, the interpretation of dark-field images must be done with great care, as common dark features of bright-field microscopy images may be invisible, and vice versa.

While the dark-field image may first appear to be a negative of the bright-field image, different effects are visible in each. In bright-field microscopy, features are visible where either a shadow is cast on the surface by the incident light or a part of the surface is less reflective, possibly by the presence of pits or scratches. Raised features that are too smooth to cast shadows will not appear in bright-field images, but the light that reflects off the sides of the feature will be visible in the dark-field images.

Use in computing

Dark-field microscopy has recently been used in computer mouse pointing devices, in order to allow an optical mouse to work on transparent glass by imaging microscopic flaws and dust on its surface.

Dark-field microscopy combined with hyperspectral imaging

When coupled to hyperspectral imaging, dark-field microscopy becomes a powerful tool for the characterization of nanomaterials embedded in cells. In a recent publication, Patskovsky et al. used this technique to study the attachment of gold nanoparticles (AuNPs) targeting CD44+ cancer cells.

Transmission electron microscope applications

Dark-field studies in transmission electron microscopy play a powerful role in the study of crystals and crystal defects, as well as in the imaging of individual atoms.

Conventional dark-field imaging

Briefly, imaging involves tilting the incident illumination until a diffracted, rather than the incident, beam passes through a small objective aperture in the objective lens back focal plane. Dark-field images, under these conditions, allow one to map the diffracted intensity coming from a single collection of diffracting planes as a function of projected position on the specimen and as a function of specimen tilt.In single-crystal specimens, single-reflection dark-field images of a specimen tilted just off the Bragg condition allow one to “light up” only those lattice defects, like dislocations or precipitates, that bend a single set of lattice planes in their neighborhood. Analysis of intensities in such images may then be used to estimate the amount of that bending. In polycrystalline specimens, on the other hand, dark-field images serve to light up only that subset of crystals that are Bragg-reflecting at a given orientation.

Weak-beam imaging

Weak-beam imaging involves optics similar to conventional dark-field, but use of a diffracted beam harmonic rather than the diffracted beam itself. Much higher resolution of strained regions around defects can be obtained in this way.

Low- and high-angle annular dark-field imaging

Annular dark-field imaging requires one to form images with electrons diffracted into an annular aperture centered on, but not including, the unscattered beam. For large scattering angles in a scanning transmission electron microscope, this is sometimes called Z-contrast imaging because of the enhanced scattering from high-atomic-number atoms.

Digital dark-field analysis

This a mathematical technique intermediate between direct and reciprocal (Fourier-transform) space for exploring images with well-defined periodicities, like electron microscope lattice-fringe images. As with analog dark-field imaging in a transmission electron microscope, it allows one to “light up” those objects in the field of view where periodicities of interest reside. Unlike analog dark-field imaging it may also allow one to map the Fourier-phase of periodicities, and hence phase gradients, which provide quantitative information on vector lattice strain.

Dark field microscopy TECHNOLOGY:

Darkfield microscopy creates contrast in transparent unstained specimens such as living cells. It depends on controlling specimen illumination so that central light which normally passes through and around the specimen is blocked. Rather than light illuminating the sample with a full cone of light (as in brightfield microscopy) the condenser forms a hollow cone with light travelling around the cone rather than through it.

This form of illumination allows only oblique rays of light to strike the specimen on the microscope stage and the image is formed by rays of light scattered by the sample and captured in the objective lens. When there is no sample on the microscope stage the view is completely dark.

Care should be taken in preparing specimens as features above and below the plane of focus can also scatter light and compromise image quality (for example, dust, fingerprints). In general, thin specimens are better because the possibility of diffraction artifacts is reduced.

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