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dark field microscopy advantages

What dark field microscopy advantages?

Limited colors (certain colors will appear, but they’re less accurate and most images will be just black and white)
Images can be difficult to interpret to those unfamiliar with dark field microscopy
Although surface details can be very apparent, the internal details of a specimen often don’t stand out as much with a dark field setup.ADVANTAGES & DISADVANTAGES.

A dark field microscope is ideal for viewing objects that are unstained, transparent and absorb little or no light.These specimens often have similar refractive indices as their surroundings, making them hard to distinguish with other illumination techniques.You can use dark field to study marine organisms such as algae and plankton, diatoms, insects, fibers, hairs, yeast and protozoa as well as some minerals and crystals, thin polymers and some ceramics.You can also use dark field in the research of live bacterium, as well as mounted cells and tissues.It is more useful in examining external details, such as outlines, edges, grain boundaries and surface defects than internal structure.

 

Advantages of darkfield microscopy:

• A dark field microscope is ideal for viewing objects that are unstained, transparent and absorb little or no light.
• These specimens often have similar refractive indices as their surroundings, making them hard to distinguish with other illumination techniques.
• You can use dark field to study marine organisms such as algae and plankton, diatoms, insects, fibers, hairs, yeast and protozoa as well as some minerals and crystals, thin polymers and some ceramics.
• You can also use dark field in the research of live bacterium, as well as mounted cells and tissues.
• It is more useful in examining external details, such as outlines, edges, grain boundaries and surface defects than internal structure.
• Dark field microscopy is often dismissed for more modern observation techniques such as phase contrast and DIC, which provide more accurate, higher contrasted images and can be used to observe a greater number of specimens.
• Recently, dark field has regained some of its popularity when combined with other illumination techniques, such as fluorescence, which widens its possible employment in certain fields.

 

It is a simple procedure which can be used on live transparent specimens, specimens which normally need to be stained (and therefore killed).
The images appear spectacular and are visually impressive.
Darkfield microscopy even allows for the visualization of objects that are below (!) the resolution of the microscope. These objects will appear as bright spots on a dark background. It is not possible to see the shape of these objects, however.

 

 

 

 

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